Journal
EXTREMOPHILES
Volume 12, Issue 6, Pages 849-854Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00792-008-0187-1
Keywords
Natronomonas pharaonis; Haloalkaliphilic; Aldehyde dehydrogenase; Reverse transcription-PCR; Molecular cloning
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Funding
- Major State Basic Research Development Program of China [2004CB719604-3]
- National Natural Science Foundation of China [30670048]
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The genome of Natronomonas pharaonis encodes genes annotated as alcohol dehydrogenase (ADH; EC 1.1.1.1) and aldehyde dehydrogenase (ALDH; EC 1.2.1.3), enzymes involved in alcohol metabolism. These genes (adh and aldH2) occur in a single copy on the chromosome. We have studied the role of these genes in ethanol metabolism in N. pharaonis. Reverse transcription-PCR analysis showed that the aldH2 gene was inducible by ethanol, but the adh gene was transcribed both in the presence and absence of ethanol. The gene encoding for ALDH of N. pharaonis (NpALDH) was cloned into a pET41a vector containing a glutathione S-transferase tag, expressed in Escherichia coli and purified by glutathione sepharose affinity chromatography. The GST-NpALDH fusion protein was cleaved by bovine enterokinase and the target enzyme showed a molecular mass of approximately 60 kDa by SDS-PAGE. The enzyme was thermophilic and alkaliphilic, the optimal temperature and pH being 60 degrees C and 8.0, respectively. NpALDH was salt independent, being most active at 0.25 M NaCl or KCl.
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