Journal
EXPERT REVIEW OF PROTEOMICS
Volume 15, Issue 10, Pages 791-800Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14789450.2018.1526679
Keywords
Phosphatidylcholines (PC); MALDI-TOF MSI; PC-related diseases
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Funding
- Deutsche Forschungsgemeinschaft (German Research Council) [DFG Schi 476/12-2, Schi 476/16-1, SFB 1052]
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Introduction: Mass spectrometry imaging (MSI) techniques are nowadays widely used to obtain spatially resolved metabolite information from biological tissues. Since (phospho)lipids occur in all animal tissues and are very sensitively detectable, they are often in the focus of such studies. This particularly applies for phosphatidylcholines (PC) which are very sensitively detectable as positive ions due to the permanent positive charge of their choline headgroup. Areas covered: After a short introduction of lipid species occurring in biological systems and approaches normally used to obtain spatially resolved mass spectra (with the focus on matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) MSI) a survey will be given which diseases have so far been characterized by changes of the PC composition. Expert commentary: Since PC species are very sensitively detectable by MS, sensitivity is not a major issue. However, spatial resolution is still limited and cellular dimensions can be hardly resolved by MALDI-TOF MSI, which is a critical point of the available approaches. Due to lacks of reproducibility and standardization further development is required.
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