Journal
EXPERT REVIEW OF PROTEOMICS
Volume 12, Issue 1, Pages 13-19Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/14789450.2015.982538
Keywords
biomarkers; cancer; clinical proteomics; mass spectrometry; quantitative proteomics; spike-in standard; stable isotope labeling; super-SILAC
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Funding
- Israel Cancer Research Fund
- Israel Science Foundation-Israeli Centers of Research Excellence (I-CORE), Gene Regulation in Complex Human Disease Center [41/11]
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Stable isotope labeling with amino acids in cell culture (SILAC) has risen as a powerful quantification technique in mass spectrometry (MS)-based proteomics in classical and modified forms. Previously, SILAC was limited to cultured cells because of the requirement of active protein synthesis; however, in recent years, it was expanded to model organisms and tissue samples. Specifically, the super-SILAC technique uses a mixture of SILAC-labeled cells as a spike-in standard for accurate quantification of unlabeled samples, thereby enabling quantification of human tissue samples. Here, we highlight the recent developments in super-SILAC and its application to the study of clinical samples, secretomes, post-translational modifications and organelle proteomes. Finally, we propose super-SILAC as a robust and accurate method that can be commercialized and applied to basic and clinical research.
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