Journal
EXPERT REVIEW OF PROTEOMICS
Volume 9, Issue 4, Pages 401-414Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.12.34
Keywords
affinity proteomics; antibody; biomarker discovery; high-throughput screening; protein microarrays; protein-protein interactions; proteomics
Categories
Funding
- EU [222635]
- AFFINOMICS [241481]
Ask authors/readers for more resources
Affinity proteomics is the field of proteome analysis based on the use of antibodies and other binding reagents as protein-specific detection probes. In this review, the particular strengths of affinity methods for determination of protein localization, functional characterization, biomarker discovery and intracellular applications, and their resulting impact in basic and clinical research are highlighted. An additional focus is on the requirements for systematic binder generation and current large-scale binder projects, including bioinformatic frameworks for epitope selection and for documentation of available binding reagents and their performance. In addition to current affinity proteomics methods and applications, including arrays of proteins, binders, lysates and tissues, approaches coupling mass spectrometry-based proteomics and affinity proteomics are reviewed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available