Journal
EXPERT REVIEW OF PROTEOMICS
Volume 8, Issue 3, Pages 335-346Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.11.15
Keywords
directed evolution; high-throughput screening; in vitro selection; mRNA display; protein domain library; protein-protein interaction; proteome library; random peptide library
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Funding
- Carolina Center for Genome Sciences
- Eshelman School of Pharmacy at the University of North Carolina at Chapel Hill (NC, USA)
- NIH [NS047650, CA119343, DA025702, CA151652]
- American Cancer Society [RSG-TBE-110472]
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mRNA display is a genotype-phenotype conjugation method that allows for amplification-based, iterative rounds of in vitro selection to be applied to peptides and proteins. mRNA display can be used to display both long natural protein and short synthetic peptide libraries with unusually high diversities for the investigation of protein-protein interactions. Here, we summarize the advantages of mRNA display by comparing it with other widely used peptide or protein-selection techniques, and discuss various applications of this technique in studying protein-protein interactions.
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