Journal
EXPERT REVIEW OF PROTEOMICS
Volume 8, Issue 5, Pages 591-604Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1586/EPR.11.53
Keywords
biomarker; clinical; histopathology; immunohistochemistry; MALDI MS imaging; mass spectrometry; prognosis; single cell
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Funding
- Amyotrophic Lateral Sclerosis Association [1856]
- Brain Science Foundation
- Daniel E Ponton Fund for the Neurosciences
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Single-cell analysis is gaining popularity in the field of mass spectrometry as a method for analyzing protein and peptide content in cells. The spatial resolution of MALDI mass spectrometry (MS) imaging is by a large extent limited by the laser focal diameter and the displacement of analytes during matrix deposition. Owing to recent advancements in both laser optics and matrix deposition methods, spatial resolution on the order of a single eukaryotic cell is now achievable by MALDI MS imaging. Provided adequate instrument sensitivity, a lateral resolution of similar to 10 mu m is currently attainable with commercial instruments. As a result of these advances, MALDI MS imaging is poised to become a transformative clinical technology. In this article, the crucial steps needed to obtain single-cell resolution are discussed, as well as potential applications to disease research.
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