Journal
EXPERT REVIEW OF PROTEOMICS
Volume 8, Issue 4, Pages 483-494Publisher
EXPERT REVIEWS
DOI: 10.1586/EPR.11.40
Keywords
CID; enrichment; ETD; HCD; neutral loss; phosphopeptide; phosphoproteomics; plasma membrane
Categories
Funding
- National Cancer Institute, National Institutes of Health [HHSN261200800001E]
- Division of Cancer Treatment and Diagnosis of the National Cancer Institute
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The response to extracellular stimuli often alters the phosphorylation state of plasma membrane-associated proteins. In this regard, generation of a comprehensive membrane phosphoproteome can significantly enhance signal transduction and drug mechanism studies. However, analysis of this subproteome is regarded as technically challenging, given the low abundance and insolubility of integral membrane proteins, combined with difficulties in isolating, ionizing and fragmenting phosphopeptides. In this article, we highlight recent advances in membrane and phosphoprotein enrichment techniques resulting in improved identification of these elusive peptides. We also describe the use of alternative fragmentation techniques, and assess their current and future value to the field of membrane phosphoproteomics.
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