Journal
EXPERT REVIEW OF PROTEOMICS
Volume 7, Issue 5, Pages 655-663Publisher
EXPERT REVIEWS
DOI: 10.1586/EPR.10.46
Keywords
mass spectrometry; multidimensional liquid chromatography; peptide fractionation; proteomics
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Funding
- 'Fundacao para a Ciencia e Tecnologia' - Portugal [SFRH/BPD/26456/2006, PTDC/SAU-NEU/103728/2008]
- Science Foundation Ireland (SFI) [03/IN351]
- SFI [04/RP1/B499]
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Peptide fractionation is extremely important in proteomics approaches. Full proteome characterization is desired from complex organisms, and with growing interest in post-translational modifications an extended protein sequence coverage is required. Peptide fractionation techniques have the great challenge of feeding current mass spectrometers in a way in which these issues are met. Peptide fractionation can be divided into three simple components: the column characteristics; the mobile phase; and peptide properties (charge, polarity, hydrophobicity and size). The current challenges are in the combination of these three components to allow comprehensive proteomics studies to be improved.
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