Journal
EXPERIMENTAL PARASITOLOGY
Volume 134, Issue 3, Pages 318-326Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2013.03.029
Keywords
Plasmodium vivax; Plasmodium falciparum; Population structure; Genetic diversity; Microsatellites
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Funding
- National Institutes of Health (NIH) [RO1 AI 075416]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [470570/2006-7]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/51199-0]
- National Institute of Allergy and Infectious Diseases (NIAID), NIH
- CNPq
- FAPESP
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Previous microsatellite analyses of sympatric populations of Plasmodium vivax and Plasmodium falciparum in Brazil revealed higher diversity in the former species. However, it remains unclear whether regional species-specific differences in prevalence and transmission levels might account for these findings. Here, we examine sympatric populations of P. vivax (n = 87) and P. falciparum (n = 164) parasites from Pursat province, Western Cambodia, where both species are similarly prevalent. Using 10 genome-wide microsatellites for P. falciparum and 13 for P. vivax, we found that the P. vivax population was more diverse than the sympatric P. falciparum population (average virtual heterozygosity [H-E], 0.87 vs. 0.66, P = 0.003), with more multiple-clone infections (89.6% vs. 47.6%) and larger mean number of alleles per marker (16.2 vs. 11.1, P = 0.07). Both populations showed significant multi-locus linkage disequilibrium suggestive of a predominantly clonal mode of parasite reproduction. The higher microsatellite diversity found in P. vivax isolates, compared to sympatric P. falciparum isolates, does not necessarily result from local differences in transmission level and may reflect differences in population history between species or increased mutation rates in P. vivax. (C) 2013 Elsevier Inc. All rights reserved.
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