Journal
EXPERIMENTAL PARASITOLOGY
Volume 128, Issue 1, Pages 61-67Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2011.02.006
Keywords
Leishmania (Leishmania) amazonensis; Synadenium carinatum; Leishmanicidal activity; Nitric oxide; Lectin
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa de Minas Gerais (Fapemig)
- Sustainable Science Institute (SSI)
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Antiparasitic effect of a lectin isolated from Synadenium carinatum latex (ScLL) was evaluated against Leishmania (Leishmania) amazonensis promastigotes/amastigotes. Pretreatment of murine inflammatory peritoneal macrophages with ScLL reduced by 65.5% the association index of macrophages and L. (L) amazonensis promastigotes. Expression of cytokines (IL-12, IL-1 and TNF-alpha) was detected in infected macrophages pretreated with ScLL (10 mu g/mL). ScLL also reduced the growth oft. (L) amazonensis amastigote intracellular forms, showing no in vitro cytotoxic effects in mammalian host cells. ScLL treatment in infected murine inflammatory peritoneal macrophages did not induce nitric oxide production, suggesting that a nitric oxide independent pathway is activated to decrease the number of intracellular Leishmania. (C) 2011 Elsevier Inc. All rights reserved.
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