Journal
EXPERIMENTAL PARASITOLOGY
Volume 129, Issue 4, Pages 337-345Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2011.09.010
Keywords
Leishmania; Virulence; Nucleotide sugar transporter; Species-specific gene; Leishmania major; Leishmania donovani
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Funding
- Canadian Institutes of Health Research
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Leishmania donovani and Leishmania infantum infections cause fatal visceral leishmaniasis, and Leishmania major causes self healing cutaneous lesions. It is poorly understood what genetic differences between these Leishmania species are responsible for the different pathologies of infection. To investigate whether L donovani species-specific genes are involved in visceral Leishmania infection, we have examined a L donovani species-specific gene Ld1590 (ortholog of LinJ15_V3.0900) that is a pseudogene in L major. We have previously shown that transgenic expression of L donovani Ld1590 in L. major significantly increased the liver and spleen parasite burdens in infected BALB/c mice. In this study we report that Ld1590 potentially encodes a nucleotide sugar transporter (NST) which localizes in the L donovani Golgi apparatus. Surprisingly, although transgenic expression of the Ld1590 NST increased L major survival in visceral organs, deletion of Ld1590 NST in L donovani had no significant effect on L. donovani survival in mice. These observations suggest that loss of the functional Ld1590 gene in L. major may have been associated with reduced virulence in visceral organs in its animal reservoir and could have contributed to L. major's tropism for cutaneous infections. (C) 2011 Elsevier Inc. All rights reserved.
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