Journal
EXPERIMENTAL PARASITOLOGY
Volume 127, Issue 1, Pages 113-118Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.07.001
Keywords
Rhipicephalus microplus; Haemaphysalis longicornis; Vaccination; Glutathione S-transferase (GST, EC 2.5.1.18); Tick
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Funding
- CNPq-Instituto Nacional de Ciencia e Tecnologia de Entomologia Molecular
- HHMI
- FINEP
- CAPES
- CNPq
- FAPERJ
- FAPERGS (Brazil)
- Ministry of Science, Culture and Education
- JSPS (Japan)
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Recombinant Glutathione S-transferase of Haemaphysalis longicornis (rGST-Hl) was expressed in Escherichia coli, purified by affinity chromatography and used in the immunization of cattle. Western blot analysis showed positive antibody response in cattle immunized with rGST-Hl. The tests also showed that immunized bovine sera recognize native Rhipicephalus micro plus proteins in different tissue extracts. Furthermore, the vaccine potential of rGST-Hl was investigated against infestation of Hereford cattle by R. microplus. Vaccination of cattle with rGST-Hl conferred partial cross-protection immunity against R. microplus. Considering the effect on number of engorged ticks, egg laying capacity and egg fertility, the overall efficacy of vaccination was of 57%, as compared with control group. (C) 2010 Elsevier Inc. All rights reserved.
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