Journal
EXPERIMENTAL PARASITOLOGY
Volume 126, Issue 4, Pages 592-602Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2010.06.031
Keywords
Trematode; Schistosoma mansoni infection; Susceptible snails; Resistant snails; Molecular mimicry; Glycoprotein; Carbohydrate epitope; Oligosaccharide mapping; Mass spectrometry
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB 535, A15]
- Osaka University (Japan)
Ask authors/readers for more resources
Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (beta 1-2)-linked xylose or terminal Fuc(alpha 1-3)GalNAc(beta 1-4)[+/- Fuc(alpha 1 -3)]GlcNAc(beta 1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host. (C) 2010 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available