Journal
EXPERIMENTAL PARASITOLOGY
Volume 124, Issue 4, Pages 442-447Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2009.12.018
Keywords
Neospora caninum; Immune response; CNS; Cytokines
Categories
Ask authors/readers for more resources
Neospora caninum causes neurologic disease in dogs and abortion in cattle. Little is known about the immune response of the CNS against this protozoan. The aim of this study was to evaluate production of IL-6, IL-10, TNF-alpha, IFN-gamma, and NO in rat mixed glial cell cultures infected by N. caninum. IFN-gamma was not observed. The mean cytorkine released after 24 and 72 h of infection were 3.8 +/- 0.6 and 3.7 +/- 0.6 pg TNF-alpha/mg protein and 2.7 +/- 0.69 and 4.1 +/- 0.64 pg IL-10/mg protein, respectively, and more than 8.0 pg IL-6/mg protein for both time points. NO levels increased 24 h post-infection (2.3 +/- 0.8 pg/mg protein) until 72 h (4.2 +/- 1.1 pg/mg protein) and the number of tachyzoites reduced with the time. Our results show high levels of regulatory cytokines that may suppress the harmful effects of IFN-gamma; high levels of TNF-alpha and NO may represent an effective response by infected glial cells against N. caninum. (C) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available