Journal
EXPERIMENTAL PARASITOLOGY
Volume 118, Issue 2, Pages 253-265Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2007.08.018
Keywords
nematode; Strongyloides stercoralis; transgenesis; vector; expression; neuron; muscle
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Funding
- NCRR NIH HHS [P40 RR002512, RR02512] Funding Source: Medline
- NIAAA NIH HHS [R01 AA017580-01, R01 AA017580] Funding Source: Medline
- NIAID NIH HHS [AI-50688, R01 AI050668-05, R01 AI050668, R01 AI022662, AI-22662] Funding Source: Medline
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Transgenesis is a valuable methodology for studying gene expression patterns and gene function. It has recently become available for research on some parasitic nematodes, including Strongyloides stereoralis. Previously, we described a vector construct, comprising the promoter and 3' UTR of the S. stercoralis gene Ss era-1 that gives expression of GFP in intestinal cells of developing F1 progeny. In the present study, we identified three new S. stercoralis promoters, which, in combination with the Ss era-1 3' UTR, can drive expression of GFP or the red fluorescent protein, mRFPmars, in tissue-specific fashion. These include Ss act-2, which drives expression in body wall muscle cells, Ss gpa-3, which drives expression in amphidial and phasmidial neurons and Ss rps-21, which drives ubiquitous expression in F1 transformants and in the gonads of microinjected P0 female worms. Concomitant microinjection of vectors containing GFP and mRFPmars gave dually transformed F1 progeny, suggesting that these constructs could be used as co-injection markers for other transgenes of interest. We have developed a vector toolkit for S. stercoralis including constructs with the Ss era-1 3' UTR and each of the promoters described above. (C) 2007 Elsevier Inc. All rights reserved.
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