Journal
EXPERIMENTAL LUNG RESEARCH
Volume 36, Issue 6, Pages 323-330Publisher
TAYLOR & FRANCIS INC
DOI: 10.3109/01902141003628579
Keywords
Caspase-8; gene expression; HIF-3 alpha; hypoxia; microarray; primary spontaneous pneumothorax
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Funding
- foundation of Changhua Christian Hospital, Taiwan [96-CCH-IRP-18]
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Primary spontaneous pneumothorax (PSP) often occurs after the rupture of small bullae or subpleural blebs in otherwise normal lungs. The underlying mechanism(s) remain unclear. The aim of this study was to identify genes potentially involved in the development of PSP. Microarray analysis was performed to identify specific gene expression patterns. Expression levels of genes identified to be significantly up-or down-regulated in association with PSP were confirmed by real-time polymerase chain reaction (qRT-PCR) and Western blotting. Immunohistochemistry was performed to identify lung cell types highly expressing these genes. Microarray analysis revealed that expression levels of hypoxia-inducible factor-3 alpha (HIF-3 alpha) and caspase-8 were significantly up-regulated in tissue from patients with PSP, whereas interferon-gamma, interleukin (IL)-6, and IL-8 were down-regulated (all P < .05). These genes are related to hypoxia, apoptosis, and inflammation. HIF-3 alpha and caspase-8 protein levels were increased in samples from patients with PSP. HIF-3 alpha and caspase-8 were localized in mesothelial cells, alveolar type II pneumocytes, and bronchoalveolar epithelial cells in samples from patients with PSP. Our findings, although obviously preliminary given the small sample size, suggest that hypoxia, inflammation, and apoptosis may play important roles in the pathogenesis of PSP.
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