Journal
EXPERIMENTAL GERONTOLOGY
Volume 54, Issue -, Pages 116-122Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2014.01.004
Keywords
Aging; B cells; Inflammation; Vaccine responses
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Funding
- NIH [AG-32576, 5R21AI096446-02, 1R21AG042826-01]
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Aging significantly decreases the influenza vaccine-specific response as we and others have previously shown. Based on our previous data in aged mice, we hypothesize that the inflammatory status of the individual and of B cells themselves would impact B cell function. We here show that the ability to generate a vaccine-specific antibody response is negatively correlated with levels of serum TNF-alpha. Moreover, human unstimulated B cells from elderly make higher levels of TNF-alpha than those from young individuals, and these positively correlate with serum TNF-alpha levels. These all negatively correlate with B cell function, measured by activation-induced cytidine deaminase, the enzyme of class switch recombination and somatic hypermutation. Only memory B cells (either IgM or switched), but not nave B cells, make appreciable levels of TNF-alpha and more in elderly as compared to young individuals. Finally, an anti-TNF-alpha antibody can increase the response in cultured B cells from the elderly, suggesting that TNF-alpha secreted by memory B cells affects IgM memory B cells and nave B cells in an autocrine and/or paracrine manner. Our results show an additional mechanism for reduced B cell function in the elderly and propose B cell-derived TNF-alpha as another predictive biomarker of in vivo and in vitro B cell responses. (c) 2014 Elsevier Inc. All rights reserved.
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