4.5 Article

Expression profiles of subtracted mRNAs during cellular senescence in human mesenchymal stem cells derived from bone marrow

Journal

EXPERIMENTAL GERONTOLOGY
Volume 48, Issue 5, Pages 464-471

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2013.02.022

Keywords

Senescence; Mesenchymal stem cells; Suppression subtractive hybridization; Differentially expressed genes; RT-PCR

Funding

  1. National Research Foundation of Korea (NRF)
  2. Ministry of Education, Science, and Technology [2012007471]

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Cellular senescence is an irreversible cell cycle arrest that limits the replicative lifespan of cells. Senescence suppresses development of tumors by regulating aging factors, such as cyclin dependent kinase inhibitor (CKI) and telomerase. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed between young human mesenchymal stem cells (Y-hMSCs) and senescent human mesenchymal stem cells (S-hMSCs). We selected positive clones that were functionally characterized by referring to public databases using NCBI BLAST tool. This search revealed that 19 genes were downregulated, and 43 genes were upregulated in S-hMSCs relative to Y-hMSCs. Among subtracted clones in Y-hMSCs, most of genes markedly were related to metabolic functions. These genes, PDIA3, WDR1, FSTL1, COPG1, LMAN1, and PDIA6, significantly downregulated. Conversely, genes for subtracted clones in S-hMSCs were mostly associated with cell adhesion. In particular, the expression levels of 9 genes, HSP90B1, EID1, ATP2B4, DDAH1, PRNP, RAB1A, PGS5, TM4SF1 and SSR3, gradually increased during senescence. These genes have not previously been identified as being related to cellular senescence, but they seemed to be potentially affected during cellular senescence. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.

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