Journal
EXPERIMENTAL GERONTOLOGY
Volume 45, Issue 7-8, Pages 596-602Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2010.01.016
Keywords
Mitochondria; Oxidative stress; Permeability; Mitochondrial matrix; Immunofluorescence microscopy; Electron microscopy; Detergents
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Funding
- Intramural NIH HHS [ZIA HL000225-32] Funding Source: Medline
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Mitochondria are a primary source as well a principal target of reactive oxygen species within cells. Using immunofluorescence microscopy, we have found that a number of mitochondrial matrix proteins are normally undetectable in formaldehyde-fixed cells permeabilized with the cholesterol-binding detergent saponin. However, exogenous or endogenous oxidative stress applied prior to fixation altered the permeability of mitochondria, rendering these matrix proteins accessible to antibodies. Electron microscopy revealed a loss of matrix density and disorganization of inner membrane cristae upon oxidative stress. Notably, the changes in permeability and in structure were rapidly reversed when the oxidative stress was relieved. The ability of reactive oxygen species to reversibly alter the permeability of the mitochondrial membrane provides a potential mechanism for communication within the cell such as between nucleus and mitochondria. Published by Elsevier Inc.
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