Journal
EXPERIMENTAL EYE RESEARCH
Volume 116, Issue -, Pages 424-432Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2013.10.013
Keywords
potassium channels; M-type K+ current; RPE; cone photoreceptors
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Funding
- NIH [EY08850, EY07703]
- RPB
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Human, monkey, and bovine retinal pigment epithelial (RPE) cells exhibit an M-type K+ current, which in many other cell types is mediated by channels composed of KCNQ alpha-subunits and KCNE auxiliary subunits. Recently, we demonstrated the expression of KCNQ1 KCNQ4, and KCNQ5 in the monkey RPE. Here, we investigated the expression of KCNQ and KCNE subunits in native bovine RPE. RT-PCR analysis revealed the expression of KCNQ1, KCNQ4, and KCNQ5 transcripts in the RPE, but, in Western blot analysis of RPE plasma membranes, only KCNQ5 was detected. Among the five members of the KCNE gene family, transcripts for KCNE1, KCNE2, KCNE3, and KCNE4 were detected in bovine RPE, but only KCNE1 and KCNE2 proteins were detected. Immunohistochemisty of frozen bovine retinal sections revealed KCNE1 expression near the apical and basal membranes of the RPE, in cone outer segments, in the outer nuclear layer, and throughout the inner retina. The localization of KCNE1 in the RPE basal membrane, where KCNQ5 was previously found to be present, suggests that this beta-subunit may contribute to M-type K+ channels in this membrane. (C) 2013 Elsevier Ltd. All rights reserved.
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