Journal
EXPERIMENTAL EYE RESEARCH
Volume 87, Issue 2, Pages 137-146Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2008.05.008
Keywords
purinergic; calcium mobilization; ocular lens
Categories
Funding
- Biotechnology and Biological Sciences Research Council (BBSRC)
- The Wellcome Trust
- The Sir Halley Stewart Trust
- National Institutes of Health [EY10558]
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Extracellular ATP has been shown to mobilize intracellular Ca2+ in cultured ovine lens epithelial cells and in human lens epithelium, suggesting a role for purines in the modulation of lens transparency. In this Study, we characterized the expression profiles of P2Y receptor isoforms throughout the rat lens at both the molecular and the functional levels. RT-PCR indicated that P2Y(1), P2Y(2), P2Y(4) and P2Y(6) are expressed in the lens, while P2Y(12), P2Y(13) and P2Y(14) are not. Immunohistochemistry, using isoform specific antibodies, indicated that the epithelium does not express P2Y, and P2Y(2), but that the underlying fiber cells, which differentiate from the epithelial cells, exhibit Strong membranous labeling. Although co-expressed in fiber cells, differences in P2Y(1) and P2Y(2) expression were apparent. P2Y(1) expression extended deeper into the lens than P2Y(2), and its expression co-localized with Cx50 gap junction plaques, while P2Y(2) did not. Labeling for P2Y(4) and P2Y(6) receptors were observed in both epithelial cells and fiber cells, but the labeling was predominantly cytoplasmic in nature. While purine agonist (ATP, ADP, UTP and UDP) application to the lens induced mobilization of intracellular Ca2+ in cortical fiber cells, little to no effect was observed in the anterior and equatorial epithelium. Thus the inability of UTP and UDP to mobilize intracellular Ca2+, in the epithelium and the predominately cytoplasmic location of P2Y(4) and P2Y(6) Suggests that these receptors may represent an inactive pool of receptors that may be activated under non-physiological conditions. In contrast, our results indicated that P2Y(1) and P2Y(2) are functionally active in fiber cells and their differential subcellular expression patterns suggest they may regulate distinct processes in the lens under steady state conditions. (C) 2008 Elsevier Ltd. All rights reserved.
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