Journal
EXPERIMENTAL DERMATOLOGY
Volume 19, Issue 8, Pages E44-E49Publisher
WILEY
DOI: 10.1111/j.1600-0625.2009.00963.x
Keywords
keratinocytes; matrix metalloproteinase-1; matrix metalloproteinase-9; toll-like receptors
Categories
Funding
- Molecular and Cellular Biodiscovery Research Program [2006-02438]
- Seoul National University Hospital Research Fund [03-2005-017-0]
- Amore Pacific Corporation
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Toll-like receptors (TLRs) on epidermal keratinocytes are the first line of defense against microbe invasion, and matrix metalloproteases (MMPs) regulate inflammation, cell migration and wound healing. In this study, we demonstrate that the mRNA and protein expressions of MMP-1 and MMP-9 in human epidermal keratinocytes are induced by ligands for TLR2, TLR3 and TLR5 [Pam3CSK4, Poly(I:C) and flagellin, respectively] in a dose-dependent manner. We also found that the ligands for TLR2, TLR3 and TLR5 activate the MAP kinases, JNK and p38 MAPK, but not ERK1/2. Furthermore, treatment with the ligands for TLR2, TLR3 and TLR5 also induced the degradation of I kappa B-alpha and activated the nuclear translocation of NF-kappa B. MMP-1 induction by the ligands for TLR2, TLR3 and TLR5 was inhibited by pretreatment with BAY11-7082 (NF-kappa B inhibitor) or SP600125 (JNK inhibitor), whereas MMP-9 expression was inhibited by pretreatment with BAY11-7082, SP600125 or SB203580. These findings demonstrate that the activation of TLR2, TLR3 or TLR5 induces the expression of MMP-1 and MMP-9 in human epidermal keratinocytes. In addition, NF-kappa B or JNK mediated the MMP-1 expression induced by TLR2, TLR3 and TLR5, whereas NF-kappa B, JNK or p38 MAPK mediated the MMP-9 expression induced by TLR2, TLR3 and TLR5.
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