Journal
EXPERIMENTAL CELL RESEARCH
Volume 327, Issue 1, Pages 91-101Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2014.05.005
Keywords
Liver fibrosis; Diagnostic probe; Type I collagen; Porphyrin
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Funding
- Department of Science and Technology (DST), Ministry of Science and Technology, Govt. of India
- Indian Council of Medical Research (ICMR, New Delhi) [GAP 0301, GAP 0383]
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Targeted molecular imaging to detect changes in the structural and functional organization of tissues, at the molecular level, is a promising approach for effective and early diagnosis of diseases. Quantitative and qualitative changes in type I collagen, which is a major component in the extra cellular matrix (ECM) of skin and other vital organs like lung, liver, heart and kidneys, are often associated with the pathophysiology of these organs. We have synthesized a fluorescent probe that comprises collagelin, a specific collagen binding peptide, coupled to fluorescent porphyrin that can effectively detect abnormal deposition of collagen in live tissues by emitting fluorescence in the near infra red (NIR) region. In this report we have presented the methodology for coupling of 5-(4-carboxy phenyl)-10, 15, 20-triphenyl porphyrin (C-TPP) to the N-terminal of collagelin or to another mutant peptide (used as a control). We have evaluated the efficacy of these fluorescent peptides to detect collagen deposition in live normal and abnormal tissues. Our results strongly suggest that porphyrin-tagged collagelin can be used as an effective probe for the non invasive in vivo detection of tissue fibrosis, especially in the liver. (C) 2014 Elsevier Inc. All rights reserved.
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