Journal
EXPERIMENTAL CELL RESEARCH
Volume 319, Issue 17, Pages 2663-2672Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2013.05.027
Keywords
Smooth muscle cells; Fluid flow shear stress; Topology; Phenotype; Arteriosclerotic plaques
Categories
Funding
- Key Basic Research Project [2011CB606204]
- National Natural Science Foundation of China [30870629]
- Fundamental Research Funds for the Central Universities [SWJTU11ZT11, SWJTU11CX054]
- Foundation of Jiangsu Provincial Key Laboratory for Interventional Medical Devices [JR1205]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
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The morphology of vascular smooth muscle cells (SMCs) in the normal physiological state depends on cytoskeletal distribution and topology beneath, and presents vertical to the direction of blood flow shear stress (FFSS) although SMCs physiologically are not directly exposed to the shear conditions of blood flow. However, this condition is relevant for arteriosclerotic plaques and the sites of a vascular stent, and little of this condition in vitro has been studied and reported till now. It is unclear what will happen to SMC morphology, phenotype and function when the direction of the blood flow changed. In this paper, the distribution of SMCs in a specific area on Ti surface was regulated by micro-strips of hyaluronic acid (HA). Cell morphology depended on the distribution of the cytoskeleton extending along the micrographic direction. Simulated vascular FFSS was perpendicular or parallel to the direction of the cytoskeleton distribution. Based on investigating the morphology, apoptotic number, phenotypes and functional factors of SMCs, it was obtained that SMCs of vertical groups showed more apoptosis, expressed more contractile types and secreted less TGF-beta 1 factor compared with SMCs of parallel groups, the number of ECs cultured by medium from SMCs of parallel groups was larger than vertical groups. This study could help to understand the effect of direction change of FFSS on patterned SMC morphology, phenotype and function. (C) 2013 Published by Elsevier Inc.
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