Journal
EXPERIMENTAL CELL RESEARCH
Volume 319, Issue 16, Pages 2490-2500Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2013.06.017
Keywords
Actin stress fibers; Response of actin cytoskeleton to stretch; Models of stress fiber kinetics
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Funding
- National Institutes of Health [5R01HL109505-03]
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The dynamic responses of actin stress fibers within a cell's cytoskeleton are central to the development and maintenance of healthy tissues and organs. Disturbances to these underlie a broad range of pathologies. Because of the importance of these responses, extensive experiments have been conducted in vitro to characterize actin cytoskeleton dynamics of cells cultured upon two-dimensional substrata, and the first experiments have been conducted for cells within three-dimensional tissue models. Three mathematical models exist for predicting the dynamic behaviors observed. Surprisingly, despite differing viewpoints on how actin stress fibers are stabilized or destabilized, all of these models are predictive of a broad range of available experimental data. Coarsely, the models of Kaunas and co-workers adopt a strategy whereby mechanical stretch can hasten the depolymerization actin stress fibers that turn over constantly, while the models of Desphande and co-workers adopt a strategy whereby mechanical stress is required to activate the formation of stress fibers and subsequently stabilize them. In three-dimensional culture, elements of both approaches appear necessary to predict observed phenomena, as embodied by the model of Lee et al. After providing a critical review of existing models, we propose lines of experimentation that might be able to test the different principles underlying their kinetic laws. (C) 2013 Elsevier Inc. All rights reserved.
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