Journal
EXPERIMENTAL CELL RESEARCH
Volume 319, Issue 20, Pages 3226-3237Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2013.07.021
Keywords
Cytoskeleton; Keratin; Monoclonal antibody; Nuclear matrix; Nuclear scaffold; Nuclear transport
Categories
Funding
- MEXT, Japan [3306: 24115512]
- MEXT, Japan
- JSPS
- Grants-in-Aid for Scientific Research [24115512, 24115003] Funding Source: KAKEN
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To uncover the molecular composition and dynamics of the functional scaffold for the nucleus, three fractions of biochemically-stable nuclear protein complexes were extracted and used as immunogens to produce a variety of monoclonal antibodies. Many helix-based cytoskeletal proteins were identified as antigens, suggesting their dynamic contribution to nuclear architecture and function. Interestingly, sets of antibodies distinguished distinct subcellular localization of a single isoform of certain cytoskeletal proteins; distinct molecular forms of keratin and actinin were found in the nucleus. Their nuclear shuttling properties were verified by the apparent nuclear accumulations under inhibition of CRM1-dependent nuclear export Nuclear keratins do not take an obvious filamentous structure, as was revealed by non-filamentous cytoplasmic keratin-specific monoclonal antibody. These results suggest the distinct roles of the helix-based cytoskeletal proteins in the nucleus. (C) 2013 Elsevier Inc. All rights reserved.
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