Journal
EXPERIMENTAL CELL RESEARCH
Volume 318, Issue 18, Pages 2365-2376Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2012.07.003
Keywords
DNA damage; Nucleolus; RNF8; BRCA1
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Funding
- Spanish Ministry of Science and Innovation [SAF2008-04136-C02-01, SAF2011-24686]
- Catalan Agency for the Administration of University and Research [AGAUR2009-SGR-1482]
- Biotechnology Reference Network (XeRBa)
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The induction of DNA double-strand breaks (DSBs) elicits a plethora of responses that redirect many cellular functions to the vital task of repairing the injury, collectively known as the DNA damage response (DDR). We have found that, in the absence of DNA damage, the DSB repair factors RNF8 and BRCA1 are associated with the nucleolus. Shortly after exposure of cells to gamma-radiation. RNF8 and BRCA1 translocated from the nucleolus to damage foci, a traffic that was reverted several hours after the damage. RNF8 interacted through its FHA domain with the ribosomal protein RPSA, and knockdown of RPSA caused a depletion of nucleolar RNF8 and BRCA1, suggesting that the interaction of RNF8 with RPSA is critical for the nucleolar localization of these DDR factors. Knockdown of RPSA or RNF8 impaired bulk protein translation, as did gamma-irradiation, the latter being partially countered by overexpression of exogenous RNF8. Our results suggest that RNF8 and BRCA1 are anchored to the nucleolus through reversible interactions with RPSA and that, in addition to its known functions in DDR, RNF8 may play a role in protein synthesis, possibly linking the nucleolar exit of this factor to the attenuation of protein synthesis in response to DNA damage. (C) 2012 Elsevier Inc. All rights reserved.
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