Journal
EXPERIMENTAL CELL RESEARCH
Volume 318, Issue 18, Pages 2324-2334Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2012.06.018
Keywords
MiR-23a; Myogenic differentiation; C2C12 myoblasts; Fast myosin heavy chain genes
Categories
Funding
- 973 National Basic Research Program of China [2009CB941602]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress the expression of their target genes post-transcriptionally. MiRNAs participate in the regulation of a variety of biological processes, including development and diseases. However, the functional role and molecular mechanism by which miRNAs regulate skeletal muscle development and differentiation are not fully understood. In this report, we identified miR-23a as a key regulator of skeletal muscle differentiation. Using bioinformatics analyses, miR-23a is predicted to target multiple adult fast myosin heavy chain (Myh) genes, including Myh 1, 2 and 4. Luciferase reporter assays show that miR-23a directly targets the 3' untranslated regions (UTRs) of these mRNAs. Interestingly, the expression level of mature miR-23a is inversely correlated with myogenic progression in mouse skeletal muscle. Both gain- and loss-of-function studies using C2C12 myoblasts demonstrate that miR-23a inhibits myogenic differentiation. These findings therefore reveal a novel role of miR-23a in regulating myogenic differentiation via inhibiting the expression of fast myosin heavy chain isoforms. (C) 2012 Elsevier Inc. All rights reserved.
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