Journal
EXPERIMENTAL CELL RESEARCH
Volume 318, Issue 20, Pages 2559-2566Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2012.08.004
Keywords
Connexin 43; Kir6.1; Ischemia preconditioning; Phosphorylation
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Funding
- Department of science and technology (DST), New Delhi [SR/FT/LS-017/2009]
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Connexin 43 (Cx43) is a phosphoprotein expressed in a wide variety of cells. Cx43 and adenosine-triphosphate-sensitive K(+)channels [K+(ATP)] are part of same signaling pathway that regulates cell survival during stress and ischemia preconditioning. Molecular mechanism for their coordinated role in ischemia/hypoxia preconditioning is not well known. Using pull down, co-immunoprecipitation assays and co-localization studies we provide evidence, for the first time that Kir6.1, a K+(ATP) channel protein component, can interact with Cx43. Further we show that the interaction was phospho-specific such that Cx43 phosphorylated at serine 262 (S262) interacted with Kir6.1 in preference to the unphosphorylated form of Cx43. Introduction of phospho-deficient mutation at serine 262 (S262A) in Cx43 completely abolished the interaction. Our data provide an interesting lead about a possible partnership between Cx43 and Kir6.1, which will help in better understanding their role in ischemia/hypoxia preconditioning. (c) 2012 Elsevier Inc. All rights reserved.
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