Journal
EXPERIMENTAL CELL RESEARCH
Volume 317, Issue 3, Pages 262-275Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2010.10.012
Keywords
Prostatic neoplasms; Tumor cells cultured; Basal epithelial cells; Tumor stem cells; Gene expression profiling
Categories
Funding
- Association pour la Recherche sur les Tumeurs de la Prostate (ARTP)
- Association pour la Recherche sur le Cancer (ARC)
- ARTP (France)
- Prostate Cancer Research Fundation (PCRF, UK)
- SALES Fundation (Argentina)
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Deciphering molecular pathways involved in the early steps of prostate oncogenesis requires both in vitro and in vivo models derived from human primary tumors. However the few recognized models of human prostate epithelial cancer originate from metastases. To date, very few models are proposed from primary tumors and immortalizing normal human prostate cells does not recapitulate the natural history of the disease. By culturing human prostate primary tumor cells onto human epithelial extra-cellular matrix, we successfully selected a new prostate cancer cell line, IGR-CaP1, and clonally-derived subclones. IGR-CaP1 cells, that harbor a tetraploid karyotype, high telomerase activity and mutated TP53, rapidly induced subcutaneous xenografts in nude mice. Furthermore, IGR-CaP1 cell lines, all exhibiting negativity for the androgen receptor and PSA, express the specific prostate markers alpha-methylacyl-CoA racemase and a low level of the prostate-specific membrane antigen PSMA, along with the prostate basal epithelial markers CK5 and CK14. More importantly, these clones express high CD44, CD133, and CXCR4 levels associated with high expression of alpha 2 beta 1-integrin and Oct4 which are reported to be prostate cancer sternness markers. RT-PCR data also revealed high activation of the Sonic Hedgehog signalling pathway in these cells. Additionally, the IGR-CaP1 cells possess a 3D sphere-forming ability and a renewal capacity by maintaining their CSC potential after xenografting in mice. As a result, the hormone-independent IGR-CaP1 cellular clones exhibit the original features of both basal prostate tissue and cancer sternness. Tumorigenic IGR-CaP1 clones constitute invaluable human models for studying prostate cancer progression and drug assessment in vitro as well as in animals specifically for developing new therapeutic approaches targeting prostate cancer stem cells. (C) 2010 Elsevier Inc. All rights reserved.
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