Journal
EXPERIMENTAL CELL RESEARCH
Volume 314, Issue 20, Pages 3692-3700Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2008.09.016
Keywords
Centrosome; CEP135; C-NAP1; Centrosome split
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Funding
- Biolmaging Research Center at GIST [R01-2007-000-20116-0]
- Korea Research Promotion Fund [KRF-2006-311-C00471]
- SRC Program [R11-2005-009-03005-0]
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A proteomic study predicted that about one hundred kinds of proteins constitute a basic structure of the centrosome. Most of the core centrosomal proteins contain extensive coiled-coil domains, suggesting that the protein-protein interaction is a Critical force for the core centrosome configuration. In the present study, we investigated a novel interaction between CEP135 and C-NAP1, two core centriolar proteins. Depletion of CEP135 caused a premature centrosome splitting. Reduction of the centrosomal C-NAP1 level was accompanied in a specific manner. Ectopic expression of the CEP135 Mutant proteins also caused centrosome splitting in association with the reduction of the centrosomal C-NAP1 levels. Based on these results, we propose that CEP135 acts as a platform protein for C-NAP1 at the centriole. (c) 2008 Elsevier Inc. All rights reserved.
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