Journal
EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 234, Issue 5, Pages 595-603Publisher
SAGE PUBLICATIONS LTD
DOI: 10.3181/0812-RM-359
Keywords
Sertoli-islet cell aggregates (SICA); rotating wall vessel (RWV) bioreactor; local immunoprotection; diabetes; islets transplantation
Categories
Funding
- National High Technology Research and Development Program of China [2003AA744051]
- Tissue Engineering Special Project of Beijing [H020920040220]
- Natural Science Foundation of China [30570498]
- NASA [NAG9-651, NCC9-130, NNJ04HC81G]
Ask authors/readers for more resources
Transplantation of pancreatic islets is a potentially attractive treatment for type I diabetes. We generated the transplantable, tissue-like aggregates composed of Sertoli cells and islets in rotating wall vessel bioreactors, SICA (Sertoli-islet cell aggregates), to improve their biological function in vitro and in vivo. The isolated islet equivalent and Sertoli cells were purified from Wistar rats and cocultured for 5 days in bioreactor to generate SICA. The SICA, islets aggregates, and fresh isolated islets were transplanted under the kidney capsule of diabetic Sprague-Dawley (SD) rats, respectively. The functions of different grafts were ascertained by blood glucose level measurements and an in vivo glucose tolerance test. In response to elevated glucose, insulin secretion from SICA was 1.4-fold higher (P < 0.05, n = 5) than islet aggregates cultured alone. Of the rats that received SICA, 90% (9/10) remained normoglycemic at 60 days post-transplantation, and the survival significantly increased compared with recipients bearing homotypic islets aggregates or freshly isolated islets. The former responded similarly with healthy rats to the glucose tolerance test. Our results support the usefulness of SICA for the treatment of type 1 diabetes without any immunosuppressive agents. Exp Biol Med 234:595-603.2009
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available