Journal
EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 234, Issue 1, Pages 63-73Publisher
SAGE PUBLICATIONS LTD
DOI: 10.3181/0805-RM-147
Keywords
proteomic analysis; autoantibodies; endothelial cell autoantigens; dengue virus
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Funding
- National Research Program for Genomic Medicine, National Science Council, Taiwan [NSC94-3112-B006-007]
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We previously showed the occurrence of autoimmune responses in dengue virus (DV) infection, which has potential implications for the pathogenesis of dengue hemorrhagic syndrome. In the present study, we have used a proteomic analysis to identify several candidate proteins on HMEC-1 endothelial cells recognized by anti-DV nonstructural protein 1 (NS1) antibodies. The target proteins, including ATP synthase beta chain, protein disulfide isomerase, vimentin, and heat shock protein 60, co-localize with anti-NS1 binding sites on nonfixed HMEC-1 cells using immunohistochemical double staining and confocal microscopy. The cross-reactivity of anti-target protein antibodies with HMEC-1 cells was inhibited by NS1 protein preabsorption. Furthermore, a cross-reactive epitope on NS1 amino acid residues 311-330 (P311-330) was predicted using homologous sequence alignment. The reactivity of dengue hemorrhagic patient sera with HMEC-1 cells was blocked by synthetic peptide P311-330 pre-absorption. Taken together, our results identify putative targets on endothelial cells recognized by anti-DV NS1 antibodies, where NSI P311-330 possesses the shared epitope. Exp Biol Med 234:63-73, 2009
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