Journal
EXPERIMENTAL AND MOLECULAR PATHOLOGY
Volume 85, Issue 2, Pages 141-145Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2008.04.005
Keywords
IGFBP-rP1; Cellular senescence; pRB; p53; Colorectal cancer
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Funding
- National Natural Science Foundation of China (NSFC) [30200333, 30570840]
- Special Research Fund of College Doctor Subject [20050335106]
- China Postdoctoral Grant [20070421181]
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Insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) is a potential tumor suppressor gene. This study attempted to explore a potential senescence-like role for IGFBP-rP1 in suppressing human colorectal cancer. Recombinant IGFBP-rP1 inhibited cell proliferation and induced G1 cell cycle arrest in RKO and CW2 cells. It induced a senescence-like phenotype by showing 2-fold higher beta-galactosidase activity in IGFBP-rP1-transfectants over that in control cells. Western blot confirmed down-regulation of phosphorylated retinoblastoma protein (pRB) and up-regulation of p53 in IGFBP-rP1-transfectants as compared with control cells. Thus, IGFBP-rP1 might be a key molecule in the cellular senescence pathway. Our results uncovered a novel molecular mechanism involving the altered expression of pRB and p53 for tumor suppressor gene IGFBP-rP1 in colorectal cancer. Restoration of IGFBP-rP1 function might have potential therapeutic significance in colorectal cancer. (C) 2008 Elsevier Inc. All rights reserved.
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