Journal
EXPERIMENTAL AND MOLECULAR MEDICINE
Volume 44, Issue 2, Pages 138-148Publisher
NATURE PUBLISHING GROUP
DOI: 10.3858/emm.2012.44.2.009
Keywords
antigens, CD40; ARHGAP35 protein, human; B-lymphocytes; CD40; cell differentiation; p190RhoGEF; plasma cells; rhoA GTP-binding protein
Funding
- Ministry for Health, Welfare & Family Affairs, Republic of Korea [A090743]
- Korea Ministry of Education
- Korea Health Promotion Institute [A090743] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Previously, we demonstrated that the p190 Rho guanine nucleotide exchange factor (p190RhoGEF) was induced following CD40 stimulation of B cells. In this study, we examined whether p190RhoGEF and a downstream effector molecule RhoA are required for B cell differentiation. Expression of p190RhoGEF positively correlated with the expression of surface markers and transcriptional regulators that are characteristic of mature B cells with plasma cell (PC) phenotypes. Moreover, either the overexpression of p190RhoGEF or the expression of a constitutively active RhoA drove cellular differentiation toward PC phenotypes. B cell maturation was abrogated in cells that overexpressed p190RhoGEF and a dominant-negative form of RhoA simultaneously. CD40-mediated maturation events were also abrogated in cells that overexpressed either dominant-negative p190RhoGEF or RhoA. Together, these data provide evidence that p190RhoGEF signaling through RhoA in CD40-activated B cells drives the induction of the PC differentiation.
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