Journal
EUROPEAN RESPIRATORY JOURNAL
Volume 45, Issue 4, Pages 1055-1065Publisher
EUROPEAN RESPIRATORY SOC JOURNALS LTD
DOI: 10.1183/09031936.00184314
Keywords
-
Categories
Funding
- Spanish Ministry of Economy and Competitiveness [SAF2011-22576]
- Sara Borrell Postdoctoral contract [CD12/00530]
- Miguel Servet Type I programme [CP13/00065]
- Miguel Servet Type II programme from the Instituto de Salud Carlos III [CP13/00023]
Ask authors/readers for more resources
We assessed whether intermittent hypoxia, which emulates one of the hallmarks of obstructive sleep apnoea (OSA), leads to altered faecal microbiome in a murine model. In vivo partial pressure of oxygen was measured in colonic faeces during intermittent hypoxia in four anesthetised mice. 10 mice were subjected to a pattern of chronic intermittent hypoxia (20 s at 5% O-2 and 40 s at room air for 6 h.day(-1)) for 6 weeks and 10 mice served as normoxic controls. Faecal samples were obtained and microbiome composition was determined by 16S rRNA pyrosequencing and bioinformatic analysis by Quantitative Insights into Microbial Ecology. Intermittent hypoxia exposures translated into hypoxia/re-oxygenation patterns in the faeces proximal to the bowel epithelium (<200 mu m). A significant effect of intermittent hypoxia on global microbial community structure was found. Intermittent hypoxia increased the alpha-diversity (Shannon index, p<0.05) and induced a change in the gut microbiota (ANOSIM analysis of beta-diversity, p<0.05). Specifically, intermittent hypoxia-exposed mice showed a higher abundance of Firmicutes and a smaller abundance of Bacteroidetes and Proteobacteria phyla than controls. Faecal microbiota composition and diversity are altered as a result of intermittent hypoxia realistically mimicking OSA, suggesting the possibility that physiological interplays between host and gut microbiota could be deregulated in OSA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available