Journal
EUROPEAN RESPIRATORY JOURNAL
Volume 37, Issue 4, Pages 933-942Publisher
EUROPEAN RESPIRATORY SOC JOURNALS LTD
DOI: 10.1183/09031936.00021110
Keywords
Acetylcholine; airway smooth muscle; dexamethasone; heat shock protein 27; p38 mitogen-activated protein kinase; mitogen-activated protein kinase phosphatase-1
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Funding
- Wellcome Trust [083905]
- China Scholarship Council
- MRC [G0700900] Funding Source: UKRI
- Medical Research Council [G0700900] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0509-10080] Funding Source: researchfish
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We determined the role of p38 mitogen-activated protein kinase (MAPK) in the increased airway smooth muscle (ASM) contractile responses following ozone and modulation by corticosteroids. Mice were exposed to air or ozone (3 ppm for 3 h) and isometric contractile responses of bronchial rings to acetylcholine (ACh) were measured using a myograph in the presence of p38 MAPK inhibitor, SB239063 (10(-6) M) or dexamethasone (10(-6) M). Because MAPK phosphatase (MKP)-1 is a negative regulator of p38 MAPK, we also studied these effects in MKP-1(-/-) mice. Bronchial rings from ozone-exposed wild-type and MKP-1(-/-) mice showed increased contractile responses, with a leftward shift of the dose-response curve in MKP-1(-/-) mice. SB239063 inhibited bronchial contraction equally in air-and ozone-exposed C57/BL6 and MKP-1(-/-) mice. Dexamethasone inhibited ACh-induced bronchial contraction in both air-and ozone-exposed C57/BL6 mice, but not in air-or ozone-exposed MKP-1(-/-) mice. ACh-stimulated p38 MAPK and heat shock protein (HSP) 27 phosphorylation, as measured by Western blotting, and this effect was suppressed by SB239063 in C57/BL6 and MKP-1(-/-) mice, but not by dexamethasone in either air-or ozone-exposed MKP-1(-/-) mice. p38 MAPK plays a role in maximal ACh-induced isometric contractile responses and increased contractility induced by ozone. Dexamethasone inhibits ACh-induced ASM contraction through phosphorylation of p38 MAPK and HSP27.
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