Journal
EUROPEAN RADIOLOGY
Volume 22, Issue 11, Pages 2328-2337Publisher
SPRINGER
DOI: 10.1007/s00330-012-2509-z
Keywords
Mesenchymal stem cells; Magnetic resonance imaging; Superparamagnetic iron oxide; Cationic; Nanovesicles; Stem cell transplantation; Ischaemic cerebrovascular disease
Funding
- National Natural Science Foundation of China [81071028, 50830107]
- Fundamental Research Funds for the Central Universities of China [09ykpy04]
- Guangdong Natural Science Foundation [9151008901000001]
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To investigate the feasibility of highly efficient and controllable stem cell labelling for cellular MRI. A new class of cationic, superparamagnetic iron oxide nanoparticle (SPION)-loaded nanovesicles was synthesised to label rat bone marrow mesenchymal stem cells without secondary transfection agents. The optimal labelling conditions and controllability were assessed, and the effect of labelling on cell viability, proliferation activity and multilineage differentiation was determined. In 18 rats, focal ischaemic cerebral injury was induced and the rats randomly injected with 1 x 10(6) cells labelled with 0-, 8- or 20-mV nanovesicles (n = 6 each). In vivo MRI was performed to follow grafted cells in contralateral striata, and results were correlated with histology. Optimal cell labelling conditions involved a concentration of 3.15 mu g Fe/mL nanovesicles with 20-mV positive charge and 1-h incubation time. Labelling efficiency showed linear change with an increase in the electric potentials of nanovesicles. Labelling did not affect cell viability, proliferation activity or multilineage differentiation capacity. The distribution and migration of labelled cells could be detected by MRI. Histology confirmed that grafted cells retained the label and remained viable. Stem cells can be effectively and safely labelled with cationic, SPION-loaded nanovesicles in a controllable way for cellular MRI. aEuro cent Stem cells can be effectively labelled with cationic, SPION-loaded nanovesicles. aEuro cent Labelling did not affect cell viability, proliferation or differentiation. aEuro cent Cellular uptake of SPION could be controlled using cationic nanovesicles. aEuro cent Labelled cells could migrate along the corpus callosum towards cerebral infarction. aEuro cent The grafted, labelled cells retained the label and remained viable.
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