Journal
EUROPEAN POLYMER JOURNAL
Volume 47, Issue 5, Pages 1090-1096Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.eurpolymj.2011.02.007
Keywords
Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Magnetic; Microspheres; DNA
Categories
Funding
- Ministry Education, Youth and Sports [2B06053]
- Grant Agency of the Czech Republic [203/09/1242, 203/09/0857, P503/10/0664]
- EU [228980]
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The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV-VIS and atomic absorption spectroscopy. The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation. Based on the highly selective recognition of streptavidin with a biotin-labeled DNA probe, DNA sensor was constructed for magnetic separation of DNA from real samples. (C) 2011 Elsevier Ltd. All rights reserved.
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