Journal
EUROPEAN POLYMER JOURNAL
Volume 46, Issue 10, Pages 1966-1974Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.eurpolymj.2010.08.005
Keywords
Electrospinning; Enzyme immobilization; Acetylcholinesterase; Styrene-maleic anhydride copolymers; Jeffamine
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Funding
- Bulgarian National Science Fund [TC-Ch-1605]
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A series of electrospun mats of styrene-maleic anhydride copolymers has been functionalized by modification with two types of spacers - a polymer with a flexible hydrophilic polyether chain (Jeffamine (R) ED) and a rigid low-molecular-weight spacer (p-phenylenediamine). The successful modification of the microfibrous mats with these bifunction compounds has been proved by ATR-FTIR and X-ray photoelectron spectroscopy (XPS) analyses. In a further step, acetylcholinesterase (AChE) has been covalently immobilized onto the modified mats using glutaraldehyde as a binding agent. The amount of bound protein and the specific activity of the immobilized AChE have been determined. The highest activity has been displayed by AChE covalently bound to Jeffamine-modified microfibrous mats. Moreover, the immobilized AChE is characterized by enhanced thermal and storage stability as compared to the free enzyme. (C) 2010 Elsevier Ltd. All rights reserve
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