Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 137, Issue 26, Pages 8380-8383Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b04806
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Funding
- Engineering and Physical Sciences Research Council (EPSRC) [EP/K034946]
- National Science Foundation (NSF) [CHE-1307021]
- JSPS Core to Core
- Leverhulme Trust
- EPSRC
- EPSRC [EP/K034936/1] Funding Source: UKRI
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1307021] Funding Source: National Science Foundation
- Engineering and Physical Sciences Research Council [1369458, EP/K034936/1] Funding Source: researchfish
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Optical spectroscopic methods do not routinely provide information on higher order hierarchical structure (tertiary/quaternary) of biological macromolecules and assemblies. This necessitates the use of time-consuming and material intensive techniques, such as protein crystallography, NMR, and electron microscopy. Here we demonstrate a spectroscopic phenomenon, superchiral polarimetry, which can rapidly characterize ligand-induced changes in protein higher order (tertiary/quaternary) structure at the picogram level, which is undetectable using conventional CD spectroscopy. This is achieved by utilizing the enhanced sensitivity of superchiral evanescent fields to mesoscale chiral structure.
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