Journal
EUROPEAN JOURNAL OF RADIOLOGY
Volume 81, Issue 1, Pages 95-99Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.ejrad.2011.03.013
Keywords
Glucose transporter; 2-Deoxy-D-glucose; Superparamagnetic iron oxide; Tumor; Magnetic resonance imaging
Funding
- National Basic Research Program of China [2011CB933503, 2007CB936002]
- National Natural Science Foundation of China [81001412]
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Glucose transporter (Glut), a cellular transmembrane receptor, plays a key role in cell glucose metabolism and is linked to a poor prognosis in various human cancers. In this study, we prepared gamma-Fe2O3 NPs coated with DMSA, in which modified with 2-DG, then gamma-Fe2O3@DMSA-DG NPs was constructed. The specific interactions between Glut1-overexpressing tumor cells (MDA-MB-231) and gamma-Fe2O3@DMSA-DG NPs were observed using Prussian blue staining and transmission electron microscope (TEM), and found that gamma-Fe2O3@DMSA-DG NPs were absorbed targetedly by the cells. Furthermore, the capacity of transporting SPIOs into tumor cells using these gamma-Fe2O3@ DMSA-DG NPs was evaluated with a 1.5 T clinical magnetic resonance imaging (MRI) scanner. It was found that the acquired MRI T2 signal intensity of MDA-MB-231cells that were treated with the gamma-Fe2O3@DMSA-DG NPs decreased significantly, and it was inhibited by competition with antibody of Glut1. Our results suggest that gamma-Fe2O3@DMSA-DG NPs are a useful targeting to Glut1-overexpressing tumor cells in vitro and that gamma-Fe2O3@DMSA-DG NPs may serve as a MRI-targeted tumor agent for better tumor imaging. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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