Impact of time between field application of Bacillus subtilis strains SB01 and SB24 and inoculation with Sclerotinia sclerotiorum on the suppression of Sclerotinia stem rot in soybean

This study evaluated the impact of time between the application of cell suspensions or cell-free filtrates of Bacillus subtilis strains SB01 or SB24 on soybean plants under field conditions and inoculation with Sclerotinia sclerotiorum on their effectiveness for suppression of S. sclerotiorum. The results showed that the cell suspensions of two strains provided greater effectiveness than the cell-free filtrates, but the suppression effectiveness decreased as the time between application in the field and S. sclerotiorum inoculation increased. The B. subtilis cell suspensions applied on soybean leaves for up to 10 days under field conditions were able to provide a significant (P < 0.01) reduction in disease severity by approximately 20-90% at 5 days after the S. sclerotiorum inoculation. When rated 15 days after S. sclerotiorum inoculation, plants treated with bacterial cells for a parts per thousand currency sign6 days reduced Sclerotinia stem rot severity by 15-70%. Most effectiveness was provided by the cell suspensions present on soybean leaves for < 3 days under field conditions, which significantly (P < 0.01) reduced disease severity by 40-70% over 15 days. In comparison, the cell-free filtrates remaining on leaves for < 6 days significantly (P < 0.01) reduced disease severity during the first 5 days after the inoculation, while the best cell-free filtrate treatments were those with a parts per thousand currency sign1-day intervals, which significantly (P < 0.01) reduced disease severity by 10-40% during 15 days after the inoculation. The effectiveness of B. subtilis was reduced when it rained after application.