Journal
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 131, Issue 1, Pages 1-7Publisher
SPRINGER
DOI: 10.1007/s10658-011-9784-4
Keywords
Apple tree infection; Erwinia amylovora; Mal d 1; Malus domestica; Pathogenesis-related protein (PR-10); Plant food allergen
Categories
Funding
- Austrian Ministry of Life [1383]
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Fireblight infection of apple and pear trees is a bacterial disease of serious economic concern. In this study, apple fruits (cultivar Elstar, Topaz) from healthy trees and trees naturally infected by the fireblight-causing bacterium Erwinia amylovora, as well as leaves from infected seedlings (cultivar Golden Delicious), were analysed for their Mal d 1 allergen concentrations. In addition, trees were treated with the two Aureobasidium pullulans biocontrol strains CF10 and CF40, active ingredients of Blossom Protect FB. Mal d 1, an 18 kDa intracellular protein is the major apple allergen in Northern and Central Europe and is a member of the family 10 of pathogenesis-related proteins, which is upregulated upon stress and pathogen attack. In young symptomless leaves from infected seedlings Mal d 1.01 transcript levels were significantly increased when compared to controls from healthy plantlets. Quantitative Mal d 1 transcript expression in field grown apple fruits did not show significant differences between samples from infected trees and controls. However, Mal d 1 protein content in apple fruits increased in fruits from infected trees. In fruits from trees with fireblight but treated with A. pullulans, Mal d 1 transcript and protein levels were reduced. These data show that an increase of Mal d 1-related allergenicity in fruits may result from fireblight infection. Thus, successful strategies against fireblight are needed to protect the orchards and simultaneously control the allergen content in fruits.
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