Journal
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 126, Issue 4, Pages 509-515Publisher
SPRINGER
DOI: 10.1007/s10658-009-9557-5
Keywords
Botrytis cinerea; Vegetable grey mould; Benzimidazole resistance; beta-tubulin gene; Double resistance
Categories
Funding
- Zhejiang Natural Science Foundation [Y3080042]
- Zhejiang Forest College [2351000971]
Ask authors/readers for more resources
From 2003 to 2006, a total of 426 single-conidial isolates of B. cinerea collected from greenhouse vegetables in China were characterized for resistance to benzimidazole fungicides and diethofencarb according to inhibition of mycelial growth. Rapid development of double-resistance to benzimidazoles and diethofencarb was observed. Three types of benzimidazole-resistant isolates, Ben R1, Ben R2 and Ben R3 were detected. A new phenotype, Ben R3, which showed low level of resistance to benzimidazole fungicides and resistance to diethofencarb, was detected with frequencies of 6.8%, 10.0%, 13.2% and 12.4% from 2003 to 2006, respectively. Further studies indicated that Ben R3 was caused by a point mutation from GAG in sensitive(S) isolates to GTG at codon 198 in the beta-tubulin gene, predicted to cause a change from glutamic acid to valine. Ben R3 isolates had comparable growth, sporulation and pathogenicity ability as isolates of other phenotypes but were more sensitive at lower temperatures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available