Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 137, Issue 27, Pages 8656-8659Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b02774
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Funding
- UC Irvine School of Physical Sciences
- National Science Foundation [CBET-1351302]
- Research Corporation (Cottrell Scholar award)
- BEST IGERT program - NSF [DGE-1144901]
- institutional Chemical and Structural Biology Training Grant predoctoral fellowship [T32-GM10856]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1351302] Funding Source: National Science Foundation
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Cell-cell interactions underlie fundamental biological processes but remain difficult to visualize over long times and large distances in tissues and live organisms. Bioluminescence imaging with luciferase-luciferin pairs is sufficiently sensitive to image cells in vivo but lacks the spatial resolution to identify cellular locations and interactions. To repurpose this technology for visualizing cellular networks, we developed a caged luciferin that produces light only when cells are in close contact. This molecule comprises a nitroaromatic core that can be selectively reduced (uncaged) by one cell type, liberating a luciferin that can be selectively consumed by neighboring, luciferase-expressing cells. When the two cell types are in contact, robust light emission is observed. This imaging strategy will enable the noninvasive visualization of cell-cell interactions relevant to organismal biology
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