Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 137, Issue 33, Pages 10444-10447Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.5b03576
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Funding
- Robert A. Welch Foundation [A-0034]
- National Institutes of Health [DK44083, DK067081]
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Comparative genomics of the bacterial thiamin pyrimidine synthase (thiC) revealed a paralogue of thiC (bzaF) clustered with anaerobic vitamin B-12 biosynthetic genes. Here we demonstrate that BzaF is a radical S-adenosylmethionine enzyme that catalyzes the remarkable conversion of aminoimidazole ribotide (AIR) to S-hydroxybenzimidazole (5-HBI). We identify the origin of key product atoms and propose a reaction mechanism. These studies represent the first step in solving a long-standing problem in anaerobic vitamin B-12 assembly and reveal an unanticipated intersection of thiamin and vitamin B-12 biosynthesis.
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