Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 730, Issue -, Pages 1-7Publisher
ELSEVIER
DOI: 10.1016/j.ejphar.2014.02.006
Keywords
Berberine Intestinal mucosal barrier; Cecal ligation and puncture (CLP); Intra-abdominal infections; Tight junction proteins
Categories
Funding
- Chinese National Natural Science Foundation [30901420, 30972880]
Ask authors/readers for more resources
NOD-like receptors play a crucial role in host defense against intestinal infection. We explored the regulatory effects of berberine on NLRs during the intestinal mucosal damaging process in rats. Male Sprague-Dawlay (SD) rats were treated with berberine for 5 d before undergoing cecal ligation and puncture (CLP) to induce polymicrobiol sepsis. The expression of nucleotide-binding oligomerization domain 2 (NOD2), NLR family-pyrin domain containing 3 (NLRP3), the activity of nuclear factor-kappa B (NF-kappa B), the levels of selected cytokines and chemokines, percentage of cell death in intestinal epithelial cells, and mucosal permeability were investigated at 0 h, 2 h, 6 h, 12 h and 24 h after CLP. Results showed that the Tumor necrosis factor-alpha (TNIF-alpha) and interleukin-6 (IL-6) level in were significantly lower in berberine treated rats compared to the control animals. The tight junction proteins level, percentage of cell death in intestinal epithelial cells and the mucosal permeability were, on the other hand, significantly elevated in berberine treated rats. The expression of NOD and NLRP3, however, were not significantly affected by berberine treatment. Our results indicate that Pretreatment with berberine attenuates tissue injury and protects the intestinal mucosal barrier in early phase of sepsis but it is likely that the mechanisms of this preventive effect do not involve the NLR pathway. (C) 2014 Elsevier B.V. All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available