Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 698, Issue 1-3, Pages 370-378Publisher
ELSEVIER
DOI: 10.1016/j.ejphar.2012.11.019
Keywords
2,3,4 ',5-tetrahydroxystilbene-2-0-beta-D glucoside; Balloon injury; Proliferation; Migration; Vascular smooth muscle cell
Categories
Funding
- Natural Science Foundation of Jiangsu Province [BK2009162]
- Natural Scoundation of the Jiangsu Higher Education Institutions of China [09KJB310010]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
2,3,4',5-tetrahydroxystilbene-2-0-beta-D glucoside (TSG) has been recognized to suppress the proliferation of vascular smooth muscle cells (VSMCs). The aim of the present study was to determine whether TSG inhibits neointimal hyperplasia in a rat carotid arterial balloon injury model. Balloon injury was induced in the left common carotid artery of rats. TSG (30, 60, 120 mg/kg/day) was treated from 3 days prior to, until 14 days after the induction of balloon injury. The ratio of intima-to-media was significantly reduced in the TSG-treated rats at 14 days after the induction of injury, which was associated with reduced expressions of proliferating cell nuclear antigen (PCNA), alpha-smooth muscle actin (alpha-SMA) and platelet-derived growth factor-BB (PDGF-BB), as markers of VSMCs proliferation and migration. Additionally, TSG significantly inhibited PDGF-BB induced cell migration in cultured VSMCs. Furthermore, we explored the underlying mechanisms for such effects of TSG. The result showed that TSG markedly reduced balloon injury-induced AKT, extracellular signal-regulated kinase (ERK1/2) and nuclear factor kappaB (NF-kappa B) activation as well as mRNA expressions of c-myc, c-fos and c-jun, which is important signal pathway for VSMCs proliferation. And in both vivo and vitro model, TSG markedly regulated matrix metalloproteinase-2, 9 expressions and collagen I, III expressions, which are key factors in extracellular matrix for VSMCs migration. These results suggest that the anti-proliferative and anti-migrative effects of TSG on VSMCs could help to explain the beneficial effects of TSG on neointima hyperplasia induced by balloon injury. (c) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available