Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 688, Issue 1-3, Pages 14-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2012.05.004
Keywords
Berberine; Colon cancer; SW480 cells; Anti-inflammatory; Apoptosis; Caspases
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Funding
- USDA-CSREES through the Vegetable & Fruit Improvement Center [2010-34402-20875]
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In the current paper, berberine, an isoquinoline alkaloid, was tested for its chemopreventive potential in colon cancer (SW480) cells. Berberine inhibited proliferation of colon cancer cells in a dose-and time-dependent manner. Interestingly, this compound exhibited minimum toxicity in normal cells at 200 mu M. Berberine arrested SW480 cell cycle at G2/M phase, which was supported by induction of p21 expression. Induction of a series of biochemical events, including loss of mitochondrial membrane potential, release of cytochrome-c to cytosol, induction of Bcl-2 family proteins, caspases and cleavage of poly (ADP-ribose) polymerase (PARP), by berberine suggests its ability to induce apoptosis. In addition, berberine also inhibited inflammation, as evidenced by induction of expression of NF kappa B and Cox(2). Furthermore, berberine inhibited caspase-8 mediated angiogenesis, as confirmed through expression of tumor necrotic factor related apoptosis-inducing ligand (TRAIL), vascular endothelial growth factor (VEGF) and survivin. The results of the current study demonstrated that berberine has the ability to cause cell cycle arrest, induce apoptosis and inhibit inflammation in colon cancer cells. The magnitude of the effects observed suggests that berberine may be worth considering for further studies of its potential applications for improving health, either as a preventative or a potential treatment. (C) 2012 Elsevier B.V. All rights reserved.
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